Escherichia coli FZβA-7

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli W2637

Escherichia coli W3110

Escherichia coli FZβA-1

Escherichia coli FZβA-2

Escherichia coli FZβA-3

Escherichia coli FZβA-4

Escherichia coli FZβA-5

Escherichia coli FZβA-6

Escherichia coli FZβA-7

Derived strains

Genotype with respect to parental

P_pET28am-P_T7-RBS-AspA

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | P_pET28am-P_T7-RBS-BtpanD-made | △akI | △akII | △akIII | P_pET28am-P_t7-RBS-PaeAspDH, △aspC | △gluDH | P_pET28am-P_T7-RBS-AspA

Bars (|) indicate differences between strains.

Modifications

Origin	Gene	Organism	Modification
Escherichia coli FZβA-1	ADC	Bacillus tequilensis	Overexpression
Escherichia coli FZβA-2	thrA	Escherichia coli	Silencing
Escherichia coli FZβA-3	metL	Escherichia coli	Silencing
Escherichia coli FZβA-4	lysC	Escherichia coli	Silencing
Escherichia coli FZβA-5	AspDH	Pseudomonas aeruginosa	Overexpression
Escherichia coli FZβA-5	aspC	Escherichia coli	Silencing
Escherichia coli FZβA-6	gdhA	Escherichia coli	Silencing
Escherichia coli FZβA-7	aspA	Escherichia coli	Overexpression

References

Xinyu Zou, Laixian Guo, Lilong Huang, Miao Li, Sheng Zhang, Anren Yang, Yu Zhang, Luying Zhu, Hongxia Zhang, Juan Zhang & Zhibin Feng (2020). Pathway construction and metabolic engineering for fermentative production of β-alanine in Escherichia coli. Applied Microbiology & Biotechnology.