Ancestors

Derived strains

• Escherichia coli JCL262
• Escherichia coli SA296

Genotype with respect to parental

ΔpflB

Genotype with respect to wild type

F⁺ (λ) | IacI^q rrnB_T14 ∆lacZ_WJ16 hsdR514 ∆araBAD_AH33 ∆rhaBAD_LD78 | F' [traD36, proAB⁺, lacI^q ZDM15] | ΔadhE, ΔldhA, ΔfrdBC, Δfnr, Δpta | ΔpflB

Bars (|) indicate differences between strains.

References

• Atsumi, Shota; Cann, Anthony F.; Connor, Michael R.; Shen, Claire R.; Smith, Kevin M.; Brynildsen, Mark P.; Chou, Katherine J.Y.; Hanai, Taizo & Liao, James C. (2008). Metabolic engineering of Escherichia coli for 1-butanol production. Metabolic Engineering, 10(6), 305-311.

• Atsumi, Shota; Li, Zhen & Liao, James C. (2009). Acetolactate Synthase from Bacillus subtilis Serves as a 2-Ketoisovalerate Decarboxylase for Isobutanol Biosynthesis in Escherichia coli. Applied and Environmental Microbiology, 75(19), 6306-6311.

• Desai, Shuchi H.; Rabinovitch-Deere, Christine A.; Tashiro, Yohei & Atsumi, Shota (2014). Isobutanol production from cellobiose in Escherichia coli. Applied Microbiology and Biotechnology, 98(8), 3727-3736.