Escherichia coli JCL262

Ancestors

Genotype with respect to wild type

F⁺ (λ) | IacI^q rrnB_T14 ∆lacZ_WJ16 hsdR514 ∆araBAD_AH33 ∆rhaBAD_LD78 | F' [traD36, proAB⁺, lacI^q ZDM15] | ΔadhE, ΔldhA, ΔfrdBC, Δfnr, Δpta | ΔpflB | Plasmid pJCL60 added | Plasmid pJCL17 added

Bars (|) indicate differences between strains.

Plasmids

• pJCL17 (P_LlacO₁::atoB(EC)-adhE2(CA))

  pJCL60 (P_LlacO₁::crt(CA)-bcd(CA)-etfAB(CA)-hbd(CA))

References

• Atsumi, Shota; Cann, Anthony F.; Connor, Michael R.; Shen, Claire R.; Smith, Kevin M.; Brynildsen, Mark P.; Chou, Katherine J.Y.; Hanai, Taizo & Liao, James C. (2008). Metabolic engineering of Escherichia coli for 1-butanol production. Metabolic Engineering, 10(6), 305-311.