Escherichia coli KW018

Ancestors

Derived strains

Genotype with respect to parental

M1-93::glk


Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | Plasmid PH5a1 added | Plasmid PH5a1 lost | ΔtrpR ΔtrpL | ΔpykF | ΔptsH | M1-37::galP | M1-93::glk

Bars (|) indicate differences between strains.


Modifications
Gene Organism Modification
glk
Overexpression

Production
Metabolites Heterologous Production type Production Biomass Carbon source Time Scale Ref.
L-tryptophan Titer 2.3 g/L Glucose Flask [ 135 ]
L-tryptophan Titer 37.0 g/L Glucose 48 h Fed-Batch [ 135 ]
L-tryptophan Volumetric productivity 1.5 g/L/h Glucose 28 h Fed-Batch [ 135 ]
L-tryptophan Substrate yield 0.17 g/g of sustrate Glucose 28 h Fed-Batch [ 135 ]
acetate Titer 3.5 g/L Glucose 46 h Fed-Batch [ 135 ]
L-tryptophan Substrate yield 0.11 g/g of sustrate Glucose Flask [ 135 ]

* Inferred from plots using RetroPlot.


References