Escherichia coli 050T4/pCTSDTQ487S-RBS55

Ancestors

All strains

Escherichia coli B0016

Escherichia coli B0016-050

Escherichia coli 050T

Escherichia coli 050T3

Escherichia coli 050T4

Escherichia coli 050T4/pCTSDTQ487S-RBS55

Genotype with respect to wild type

Δack-pta ΔpflB ΔadhE ΔfrdA ΔldhA | ilvE::T7RNAP | ΔleuA | P_pntA::P_T7 | Plasmid pCTSDTQ487S-RBS55 added

Bars (|) indicate differences between strains.

Plasmids

pCTSDTQ487S-RBS55 (P_T7-ilvC-T_T7-P_T7-RBS55-alsSQ487S-P_T7-ilvD)

Production

Metabolites	Heterologous	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
3-methyl-2-oxobutanoate		Titer	19.2 g/L		Glucose		Flask	[ 221 ]
isobutanol		Titer	0.57 g/L		Glucose		Flask	[ 221 ]

* Inferred from plots using RetroPlot.

References

Li Zhou, Ying Zhu, Zhongzhe Yuan, Guangqing Liu, Zijin Sun, Shiyu Du, He Liu, Yating Li, Haili Liu, and Zhemin Zhou (2022). Evaluation of Metabolic Engineering Strategies on 2-Ketoisovalerate Production by Escherichia coli. Applied and Environmental Microbiology. 88(17): e00976-22.