Escherichia coli W-H18/pM2/pR1

Ancestors

Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlysA ΔthrB ΔmetA ΔlacI | ΔldhA ΔadhE ΔpflB | ΔptsG | ΔPgalP::Plac | ΔPppc::Ptrc | ΔiclR ΔarcA | ΔPaspA::Ptrc | ΔPglk::Plac | ΔPasd::Ptrc | ΔgalP::glfZm | Plasmid pR1 added | Plasmid pM2 added

Bars (|) indicate differences between strains.


Plasmids

Production
Metabolites Heterologous Production type Production Biomass Carbon source Time Scale Ref.
L-homoserine Titer 12.3 g/L Glucose Flask [ 238 ]
L-homoserine Titer 12.5 g/L Glucose Flask [ 238 ]
L-homoserine Substrate yield 0.68 mol/mol of substrate Glucose Flask [ 238 ]
L-homoserine Substrate yield 0.68 mol/mol of substrate Glucose Flask [ 238 ]
L-homoserine Volumetric productivity 0.59 g/L/h Glucose Flask [ 238 ]
L-homoserine Volumetric productivity 0.73 g/L/h Glucose Flask [ 238 ]
L-homoserine Titer 110.8 g/L Glucose Fed-Batch [ 238 ]
L-homoserine Volumetric productivity 1.82 g/L/h Glucose Fed-Batch [ 238 ]
L-homoserine Substrate yield 0.96 mol/mol of substrate Glucose Fed-Batch [ 238 ]

* Inferred from plots using RetroPlot.


References