Silencing
Overexpression
Feedback insensitive
Attenuation
Feed-forward insensitive
Inhibition insensitive
Unstated
Combined modifications
Titer in Flask for indigo
| Modifications | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Strain | Maximum production | fmoMa | cFMOCg | tnaAEc | trpREc | aroGEc | trpEEc | aroLEc | pykFEc | pykAEc | tktAEc |
| E. coli W3110 | 0 mg/L | ||||||||||
| E. coli W3110/pTrc-cfmo | 10.1 mg/L | ||||||||||
| E. coli W3110/pTrc-fmo | 19 mg/L | ||||||||||
| E. coli W3110/pTrcTF | 35.5 mg/L | ||||||||||
| E. coli W3110/pTrcTF1 | 39.3 mg/L | ||||||||||
| E. coli IND2/pTrcTF1 | 44.2 mg/L | ||||||||||
| E. coli IND2/pTrcTF1/pTac-GE2 | 60.4 mg/L | ||||||||||
| E. coli IND2/pTrcTF1/pTac-G | 60.8 mg/L | ||||||||||
| E. coli IND2/pTrcTF1/pTac-GE1 | 64.9 mg/L | ||||||||||
| E. coli IND2/pTrcTF1/pTac-GEL | 90.8 mg/L | ||||||||||
| E. coli IND4/pTrcTF1/pTac-GEL | 104 mg/L | ||||||||||
| E. coli IND5/pTrcTF1/pTac-GEL | 108 mg/L | ||||||||||
Titer in Fed-Batch for indigo
| Modifications | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Strain | Maximum production | trpREc | pykFEc | pykAEc | trpEEc | aroGEc | aroLEc | tnaAEc | fmoMa | tktAEc |
| E. coli IND4/pTrcTF1/pTac-GEL | 629 mg/L | |||||||||
| E. coli IND5/pTrcTF1/pTac-GEL | 640 mg/L | |||||||||