Silencing
Overexpression
Feedback insensitive
Attenuation
Feed-forward insensitive
Inhibition insensitive
Unstated
Combined modifications

Titer in Flask for L-alanine

Modifications
Strain Maximum production aspCEc ppcCg pykAEc pykFEc alaDGs ptaEc ldhAEc adhEEc pflBEc gapAEc alaEEc lipAEc atpAEc dadXEc ackAEc frdAEc
E. coli B0016-060B 20 mg/L
E. coli XA00 240.5538 mg/L
E. coli XA01 358.15788 mg/L
E. coli XA02 458.8341 mg/L
E. coli W1485lip2/pHSGADH 850 mg/L
E. coli TBLA-1/pUCADH 1,000 mg/L
E. coli W1485lip2/pUCADH 1,300 mg/L
E. coli B0016-060BC 1,560 mg/L
E. coli W1485lip2/pSTVADH 2,300 mg/L
E. coli TBLA-1/pHSGADH 3,000 mg/L
E. coli TBLA-1/pSTVADH 3,600 mg/L
E. coli W-125 11,800 mg/L
E. coli W-126 16,300 mg/L
E. coli W-126-G 18,600 mg/L
E. coli W-126-GA(L) 22,800 mg/L
E. coli W-126-GA(M) 24,800 mg/L
E. coli W-126-GA(H) 24,900 mg/L

Titer in Batch for L-alanine

Modifications
Strain Maximum production ldhAEc alaDGs mgsAEc dadXEc pflBEc frdAEc frdBEc frdCEc frdDEc adhEEc ackAEc ptaEc gapAEc alaEEc betTEc ppsAEc poxBEc aceEEc aceFEc alaDLf lipAEc atpAEc eutDEc pdhRSd sgrTEc ptsGEc lpdEc aldBs aldBc aldMs
E. coli BL21(DE3)/pN-3 6,390 mg/L
E. coli BL21(DE3)/pN-4 6,520 mg/L
E. coli BL21(DE3)/pN-1 7,190 mg/L
E. coli BL21(DE3)/pN-2 7,810 mg/L
E. coli W1485lip2/pSTVADH 10,900 mg/L
E. coli TBLA-1/pSTVADH 14,700 mg/L
E. coli XZ111 16,126.014 mg/L
E. coli TBLA-1ΔldhA/pSTVADH 20,000 mg/L
E. coli ALS929 pTrc99A-alaD 34,000 mg/L
E. coli XZ113 43,032.402 mg/L
E. coli XZ123 74,126.208 mg/L
E. coli XZ129 74,215.302 mg/L
E. coli XZ115 74,482.584 mg/L
E. coli XZ130 75,462.618 mg/L
E. coli XZ131 95,241.486 mg/L
E. coli W-126-GA(M) 102,500 mg/L
E. coli XZ132 113,951.226 mg/L
E. coli B0016-060BC 120,800 mg/L
E. coli W-BetT 121,400 mg/L
E. coli W-PAla-7-BetT 134,500 mg/L
E. coli B0016-090BC 195,200 mg/L

Titer in Fed-Batch for L-alanine

Modifications
Strain Maximum production pflBEc ppsAEc poxBEc aceEEc aceFEc ldhAEc alaDLf dadXEc alaDGs ackAEc ptaEc adhEEc frdAEc eutDEc mgsAEc pdhRSd sgrTEc ptsGEc frdBEc frdCEc aldBc fadREc tyrTEc
E. coli ALS887 pTrc99A-alaD 32,000 mg/L
E. coli M-6 80,460 mg/L
E. coli ALS929 pTrc99A-alaD 88,000 mg/L
E. coli B0016-060BC 167,700 mg/L
E. coli B0016-080BC 182,600 mg/L

Substrate yield in Batch for L-alanine

Modifications
Strain Maximum production ldhAEc alaDGs mgsAEc dadXEc pflBEc frdAEc frdBEc frdCEc frdDEc adhEEc ackAEc ptaEc gapAEc alaEEc betTEc ppsAEc poxBEc aceEEc aceFEc alaDLf lipAEc atpAEc eutDEc pdhRSd sgrTEc ptsGEc lpdEc aldBs aldBc aldMs
E. coli W1485lip2/pSTVADH 0.11028 g/g of sustrate
E. coli TBLA-1/pSTVADH 0.14836 g/g of sustrate
E. coli TBLA-1ΔldhA/pSTVADH 0.20177 g/g of sustrate
E. coli BL21(DE3)/pN-3 0.3195 g/g of sustrate
E. coli BL21(DE3)/pN-4 0.326 g/g of sustrate
E. coli BL21(DE3)/pN-1 0.3595 g/g of sustrate
E. coli BL21(DE3)/pN-2 0.3905 g/g of sustrate
E. coli XZ111 0.81 g/g of sustrate
E. coli W-126-GA(M) 0.84 g/g of sustrate
E. coli XZ113 0.86 g/g of sustrate
E. coli ALS929 pTrc99A-alaD 0.86 g/g of sustrate
E. coli W-BetT 0.87 g/g of sustrate
E. coli B0016-060BC 0.883 g/g of sustrate
E. coli B0016-090BC 0.886 g/g of sustrate
E. coli XZ115 0.93 g/g of sustrate
E. coli XZ123 0.93 g/g of sustrate
E. coli XZ129 0.93 g/g of sustrate
E. coli XZ130 0.93 g/g of sustrate
E. coli XZ132 0.95 g/g of sustrate
E. coli XZ131 0.95 g/g of sustrate

Substrate yield in Flask for L-alanine

Modifications
Strain Maximum production alaDGs ptaEc ldhAEc adhEEc pflBEc gapAEc alaEEc dadXEc ackAEc frdAEc
E. coli B0016-060BC 0.295 g/g of sustrate
E. coli W-125 0.44 g/g of sustrate
E. coli W-126 0.49 g/g of sustrate
E. coli W-126-G 0.57 g/g of sustrate
E. coli W-PAla-7-BetT 0.89 g/g of sustrate
E. coli B0016-060B 2 g/g of sustrate

Substrate yield in Fed-Batch for L-alanine

Modifications
Strain Maximum production pflBEc ppsAEc poxBEc aceEEc aceFEc ldhAEc alaDLf eutDEc mgsAEc pdhRSd sgrTEc ptsGEc ackAEc ptaEc adhEEc frdAEc dadXEc alaDGs frdBEc frdCEc aldBc fadREc tyrTEc
E. coli ALS887 pTrc99A-alaD 0.63 g/g of sustrate
E. coli B0016-080BC 0.863 g/g of sustrate
E. coli ALS929 pTrc99A-alaD 1 g/g of sustrate
E. coli M-6 1.02 g/g of sustrate

Volumetric productivity in Batch for L-alanine

Modifications
Strain Maximum production alaDGs ptaEc ldhAEc adhEEc pflBEc gapAEc alaEEc betTEc eutDEc mgsAEc pdhRSd sgrTEc ptsGEc lpdEc ackAEc frdAEc dadXEc
E. coli B0016-090BC 3,070 mg/L/h
E. coli B0016-060BC 3,090 mg/L/h
E. coli W-126-GA(M) 4,270 mg/L/h
E. coli W-BetT 5,060 mg/L/h
E. coli W-PAla-7-BetT 5,600 mg/L/h

Volumetric productivity in Fed-Batch for L-alanine

Modifications
Strain Maximum production pflBEc ppsAEc poxBEc aceEEc aceFEc ldhAEc alaDLf dadXEc alaDGs ackAEc ptaEc adhEEc frdAEc eutDEc mgsAEc pdhRSd sgrTEc ptsGEc
E. coli B0016-060BC 2,960 mg/L/h
E. coli B0016-080BC 3,070 mg/L/h
E. coli ALS929 pTrc99A-alaD 4,000 mg/L/h

Biomass-specific yield in Flask for L-alanine

Modifications
Strain Maximum production alaDGs ptaEc ldhAEc adhEEc pflBEc gapAEc alaEEc
E. coli W-126-GA(H) 3.26 mg/g DCW
E. coli W-126-GA(M) 3.41 mg/g DCW
E. coli W-126-GA(L) 6 mg/g DCW
E. coli W-126-G 13.3 mg/g DCW