Lina Liu, Sheng Chen, Jing Wu, Phosphoenolpyruvate:glucose phosphotransferase system modification increases the conversion rate during l-tryptophan production in Escherichia coli, Journal of Industrial Microbiology and Biotechnology, Volume 44, Issue 10, 1 October 2017, Pages 1385–1395.

Strains

• Escherichia coli ΔpykA
• Escherichia coli ΔpykF
• Escherichia coli ΔpykF/pykA
• Escherichia coli ΔpykF/ptsH
• Escherichia coli JM109

Plasmids

• pSTV-03
• pMD18-T
• pMD18-ptsHN12S

Metabolite production

• L-tryptophan
  Titer
  • 0.78 g/L (Escherichia coli ΔpykF/ptsH in Flask)
  • 3.8 g/L (Escherichia coli ΔpykF/ptsH in Fed-Batch)
  • 2.43 g/L (Escherichia coli ΔpykF in Flask)
  • 45.5 g/L (Escherichia coli ΔpykF in Fed-Batch)
  • 2.25 g/L (Escherichia coli ΔpykA in Flask)
  • 1.37 g/L (Escherichia coli ΔpykF/pykA in Batch)
  Substrate yield
  • 0.128 g/g of sustrate (Escherichia coli ΔpykF/ptsH in Flask)
  • 0.073 g/g of sustrate (Escherichia coli ΔpykF/ptsH in Fed-Batch)
  • 0.065 g/g of sustrate (Escherichia coli ΔpykF in Flask)
  • 0.14 g/g of sustrate (Escherichia coli ΔpykF in Fed-Batch)
  • 0.056 g/g of sustrate (Escherichia coli ΔpykA in Flask)
  • 0.051 g/g of sustrate (Escherichia coli ΔpykF/pykA in Batch)
• acetate
  Titer
  • 0.97 g/L (Escherichia coli ΔpykF/ptsH in Flask)
  • 0.2 g/L (Escherichia coli ΔpykF/ptsH in Fed-Batch)
  • 6.79 g/L (Escherichia coli ΔpykF in Flask)
  • 1.9 g/L (Escherichia coli ΔpykF in Fed-Batch)
  • 7.53 g/L (Escherichia coli ΔpykA in Flask)
  • 4.25 g/L (Escherichia coli ΔpykF/pykA in Flask)