Kaemwich Jantama, M.J. Haupt, Spyros A. Svoronos, Xueli Zhang, J.C. Moore, K.T. Shanmugam & L.O. Ingram (2007). Combining metabolic engineering and metabolic evolution to develop nonrecombinant strains of Escherichia coli C that produce succinate and malate. Biotechnology & Bioengineering.

Strains

• Escherichia coli KJ012
• Escherichia coli KJ017
• Escherichia coli KJ032
• Escherichia coli KJ060
• Escherichia coli KJ070
• Escherichia coli KJ071
• Escherichia coli KJ072
• Escherichia coli KJ073
• Escherichia coli C

Metabolite production

• (S)-malate
  Titer
  • 39.0 mM (Escherichia coli KJ060 in Batch)
  • 516.0 mM (Escherichia coli KJ071 in Batch)
  • 118.0 mM (Escherichia coli KJ073 in Batch)
  • 0.0 mM (Escherichia coli C in Batch)
  • 0.0 mM (Escherichia coli KJ012 in Batch)
  • 0.0 mM (Escherichia coli KJ017 in Batch)
• pyruvate
  Titer
  • 0.0 mM (Escherichia coli KJ060 in Batch)
  • 58.0 mM (Escherichia coli KJ071 in Batch)
  • 55.0 mM (Escherichia coli KJ073 in Batch)
  • 0.0 mM (Escherichia coli KJ012 in Batch)
  • 0.0 mM (Escherichia coli KJ017 in Batch)
  • 33.0 mM (Escherichia coli C in Batch)
• acetate
  Titer
  • 250.0 mM (Escherichia coli KJ060 in Batch)
  • 64.0 mM (Escherichia coli KJ071 in Batch)
  • 183.0 mM (Escherichia coli KJ073 in Batch)
  • 152.0 mM (Escherichia coli C in Batch)
  • 26.0 mM (Escherichia coli KJ012 in Batch)
  • 181.0 mM (Escherichia coli KJ017 in Batch)
• (R)-lactate
  Titer
  • 2.0 mM (Escherichia coli KJ060 in Batch)
  • 0.0 mM (Escherichia coli KJ071 in Batch)
  • 0.0 mM (Escherichia coli KJ073 in Batch)
  • 98.0 mM (Escherichia coli C in Batch)
  • 0.0 mM (Escherichia coli KJ012 in Batch)
  • 38.0 mM (Escherichia coli KJ017 in Batch)
• formate
  Titer
  • 0.0 mM (Escherichia coli KJ060 in Batch)
  • 0.0 mM (Escherichia coli KJ071 in Batch)
  • 0.0 mM (Escherichia coli KJ073 in Batch)
  • 262.0 mM (Escherichia coli C in Batch)
  • 0.0 mM (Escherichia coli KJ012 in Batch)
  • 199.0 mM (Escherichia coli KJ017 in Batch)
• succinate
  Substrate yield
  • 0.19 mol/mol of substrate (Escherichia coli C in Batch)
  • 0.12 g/g of sustrate (Escherichia coli C in Batch)
  • 0.2 mol/mol of substrate (Escherichia coli KJ012 in Batch)
  • 0.13 g/g of sustrate (Escherichia coli KJ012 in Batch)
  • 0.74 mol/mol of substrate (Escherichia coli KJ017 in Batch)
  • 0.49 g/g of sustrate (Escherichia coli KJ017 in Batch)
  • 0.92 g/g of sustrate (Escherichia coli KJ060 in Batch)
  • 1.41 mol/mol of substrate (Escherichia coli KJ060 in Batch)
  • 0.78 mol/mol of substrate (Escherichia coli KJ071 in Batch)
  • 0.53 g/g of sustrate (Escherichia coli KJ071 in Batch)
  • 1.2 mol/mol of substrate (Escherichia coli KJ073 in Batch)
  • 0.77 g/g of sustrate (Escherichia coli KJ073 in Batch)
  Titer
  • 49.0 mM (Escherichia coli C in Batch)
  • 6.0 mM (Escherichia coli KJ012 in Batch)
  • 288.0 mM (Escherichia coli KJ017 in Batch)
  • 733.0 mM (Escherichia coli KJ060 in Batch)
  • 280.0 mM (Escherichia coli KJ071 in Batch)
  • 668.0 mM (Escherichia coli KJ073 in Batch)