Escherichia coli HW4-2

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli W2637

Escherichia coli W3110

Escherichia coli HW1

Escherichia coli HW1-5

Escherichia coli HW2-1

Escherichia coli HW2-2

Escherichia coli HW2-4

Escherichia coli HW3-1

Escherichia coli HW3-4

Escherichia coli HW4-1

Escherichia coli HW4-2

Derived strains

Escherichia coli HW4-3

Genotype with respect to parental

rph::P_trc-purH

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | △lacI, hisL::Ptrc | △hisG, tdcD::P_trc-hisG_Cg^S143F | ylbE::P_trc-hisG_Cg^S143F | ilvG::P_trc-hisG_Cg^S143F | yghX::P_trc-hisD-hisC-hisB-hisH-hisA-hisF-hisI | trpR::prsA, △purR | yeeP::P_trc-zwf, yjiV::P_trc-gnd | △mazG, △ushA, △surE, △yjjG, △yrfG, △amn, △ygdH | rph::P_trc-purH

Bars (|) indicate differences between strains.

Modifications

Gene	Organism	Modification
purH	Escherichia coli	Overexpression
rph	Escherichia coli	Silencing

Production

Metabolites	Heterologous	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
L-histidine		Titer	5.6 g/L	24.2 OD₆₀₀	Glucose		Flask	[ 203 ]

* Inferred from plots using RetroPlot.

References

Heyun Wu, Daoguang Tian, Xiaoguang Fan, Weiming Fan, Yue Zhang, Shuai Jiang, Chenhui Wen, Qian Ma, Ning Chen & Xixian Xie (2020). Highly Efficient Production of l‑Histidine from Glucose by Metabolically Engineered Escherichia coli. ACS Synthetic Biology.