Escherichia coli 050TY/pCTSDTQ487S-RBS55

Ancestors

Genotype with respect to wild type
Δack-pta ΔpflB ΔadhE ΔfrdA ΔldhA | ilvE::T7RNAP | ΔleuA | PpntA::PT7 | aceF-TagDAS+4 | Plasmid pCTSDTQ487S-RBS55 added

Bars (|) indicate differences between strains.


Plasmids

Production
Metabolites Heterologous Production type Production Biomass Carbon source Time Scale Ref.
3-methyl-2-oxobutanoate Titer 55.8 g/L Glucose Fed-Batch [ 221 ]
3-methyl-2-oxobutanoate Volumetric productivity 2.14 g/L/h Glucose Fed-Batch [ 221 ]
3-methyl-2-oxobutanoate Specific production rate 0.162 g/g DCW/h Glucose Fed-Batch [ 221 ]
3-methyl-2-oxobutanoate Substrate yield 0.852 mol/mol of substrate Glucose Fed-Batch [ 221 ]
isobutanol Substrate yield 0.036 mol/mol of substrate Glucose Fed-Batch [ 221 ]
pyruvate Substrate yield 0.022 mol/mol of substrate Glucose Fed-Batch [ 221 ]
succinate Substrate yield 0.04 mol/mol of substrate Glucose Fed-Batch [ 221 ]
acetate Substrate yield 0.134 mol/mol of substrate Glucose Fed-Batch [ 221 ]
3-methyl-2-oxobutanoate Titer 22.0 g/L Glucose Flask [ 221 ]
3-methyl-2-oxobutanoate Substrate yield 0.94 mol/mol of substrate Glucose Flask [ 221 ]

* Inferred from plots using RetroPlot.


References