Liu S, Wang BB, Xu JZ, Zhang WG. Engineering of Shikimate Pathway and Terminal Branch for Efficient Production of L-Tryptophan in Escherichia coli. Int J Mol Sci. 2023 Jul 24;24(14):11866.

Strains

• Escherichia coli T8
• Escherichia coli T8B
• Escherichia coli T8D
• Escherichia coli T8E
• Escherichia coli T8K
• Escherichia coli T8A
• Escherichia coli T8C
• Escherichia coli T9
• Escherichia coli T11
• Escherichia coli T12
• Escherichia coli T13
• Escherichia coli T14
• Escherichia coli T15
• Escherichia coli T10
• Escherichia coli JM109

Plasmids

• pSB4K5
• pSB4K5-aroB
• pSB4K5-aroD
• pSB4K5-aroE
• pSB4K5-aroK
• pSB4K5-aroA
• pSB4K5-aroC

Metabolite production

• L-tyrosine
  Titer
  • 0.9 g/L (Escherichia coli T12 in Flask)
• L-phenylalanine
  Titer
  • 1.65 g/L (Escherichia coli T12 in Flask)
• anthranilate
  Titer
  • 1.87 g/L (Escherichia coli T12 in Flask)
  • 2.07 g/L (Escherichia coli T11 in Flask)
• L-tryptophan
  Titer
  • 9.7 g/L (Escherichia coli T8 in Flask)
  • 11.27 g/L (Escherichia coli T8E in Flask)
  • 10.16 g/L (Escherichia coli T8K in Flask)
  • 11.85 g/L (Escherichia coli T9 in Flask)
  • 12.61 g/L (Escherichia coli T10 in Flask)
  • 13.23 g/L (Escherichia coli T11 in Flask)
  • 53.65 g/L (Escherichia coli T13 in Fed-Batch)
  • 14.12 g/L (Escherichia coli T13 in Flask)
  • 12.29 g/L (Escherichia coli T14 in Flask)
  • 12.71 g/L (Escherichia coli T15 in Flask)
  Substrate yield
  • 0.129 g/g of sustrate (Escherichia coli T8 in Flask)
  • 0.133 g/g of sustrate (Escherichia coli T8E in Flask)
  • 0.131 g/g of sustrate (Escherichia coli T8K in Flask)
  • 0.14 g/g of sustrate (Escherichia coli T9 in Flask)
  • 0.145 g/g of sustrate (Escherichia coli T10 in Flask)
  • 0.149 g/g of sustrate (Escherichia coli T11 in Flask)
  • 0.238 g/g of sustrate (Escherichia coli T13 in Fed-Batch)