Escherichia coli XAR31

Ancestors

All strains

Escherichia coli K12

Escherichia coli BW25113

Escherichia coli BW25113/F'

Escherichia coli XY01

Escherichia coli XY02

Escherichia coli XY04

Escherichia coli XY11

Escherichia coli XY21

Escherichia coli XY22

Escherichia coli XY28

Escherichia coli XY31

Escherichia coli XAR31

Genotype with respect to wild type

F⁺ (λ) | IacI^q rrnB_T14 ∆lacZ_WJ16 hsdR514 ∆araBAD_AH33 ∆rhaBAD_LD78 | F׳ proAB lacI^qZΔM15 Tn10 (Tet^r) | ΔompT::P_tac-ppc_Cg | ΔpykA ΔpykF | Δmdh | ΔaspA | ΔgalR, ptsG::Ptac-glk | ΔfrdABCD | frdABCD::P₁₁₉-bt-ca,pykA::P₁₁₉-Mspck, poxB::P₁₁₉-acs | ldhA::P_ldhA-aspC_Cg-rocG_Bs | Plasmid pLB1a-CR added

Bars (|) indicate differences between strains.

Plasmids

pLB1a-CR (P_araBAD-aspC_Ec rocG_Bs)

Production

Metabolites	Heterologous	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
L-aspartate		Titer	249.0 mM		Glucose	21 h	Fed-Batch	[ 223 ]
L-aspartate		Substrate yield	0.53 mol/mol of substrate		Glucose	21 h	Fed-Batch	[ 223 ]

* Inferred from plots using RetroPlot.

References

Xiaoyu Piao, Lei Wang, Baixue Lin, Hao Chen, Weifeng Liu & Yong Tao (2019). Metabolic engineering of Escherichia coli for production of L-aspartate and its derivative β-alanine with high stoichiometric yield. Metabolic Engineering.