Silencing
Overexpression
Feedback insensitive
Attenuation
Feed-forward insensitive
Inhibition insensitive
Unstated
Combined modifications

Titer in Flask for L-phenylalanine

Modifications
Strain Maximum production aroGEc aroFEc pheAEc tyrREc tyrAEc trpEEc csrAEc csrDEc csrBEc tktAEc zwfEc ptsHEc ptsIEc crrEc galPEc glkEc ydiBEc aroCEc aroDEc ppsAEc pckEc talBEc
E. coli JM101-fbr 100 mg/L
E. coli PB12-fbr 160 mg/L
E. coli Xllp02 770 mg/L
E. coli Xllp12 1,180 mg/L
E. coli NST37 1,194.87 mg/L
E. coli NST [pTktA] 1,217.11 mg/L
E. coli NST [pZwf] 1,265.75 mg/L
E. coli NSTCSRACSRD 1,415.42 mg/L
E. coli NSTCSRA [pCsrB] 1,521.57 mg/L
E. coli NSTCSRA [pZwf] 1,606 mg/L
E. coli NSTCSRD 1,612.07 mg/L
E. coli NSTCSRA 1,669.84 mg/L
E. coli Xllp15 1,680 mg/L
E. coli Xllp14 1,740 mg/L
E. coli NSTCSRA [pTkaFpA] 1,840 mg/L
E. coli Xllp18 1,860 mg/L
E. coli NST [pCsrB] 2,334.73 mg/L
E. coli NSTCSRA [pTktA] 2,391.5 mg/L
E. coli Xllp09 2,690 mg/L
E. coli Xllp03 2,870 mg/L
E. coli Xllp10 2,980 mg/L
E. coli HD-1 3,060 mg/L
E. coli Xllp06 3,260 mg/L
E. coli Xllp19 3,280 mg/L
E. coli Xllp11 3,340 mg/L
E. coli Xllp05 3,650 mg/L
E. coli Xllp17 3,670 mg/L
E. coli Xllp13 3,690 mg/L
E. coli Xllp04 4,200 mg/L
E. coli Xllp07 4,210 mg/L
E. coli Xllp20 4,770 mg/L
E. coli Xllp23 6,120 mg/L
E. coli Xllp08 6,310 mg/L
E. coli Xllp22 6,430 mg/L
E. coli Xllp21 7,150 mg/L
E. coli Xllp16 7,430 mg/L

Titer in Fed-Batch for L-phenylalanine

Modifications
Strain Maximum production tyrREc pheAEc aroFEc ptsHEc galPEc glkEc aroDEc dadXEc cvrAEc aroEEc xfpkBa ylbEEc pycCg pykFEc dhaLEc pckEc aspAEc mhpAEc adhEEc thrWEc ykfNEc
E. coli HD-1 45,000 mg/L
E. coli Xllp21 72,900 mg/L
E. coli PHE07 74,010 mg/L
E. coli PHE08 77,150 mg/L
E. coli PHE09 80,840 mg/L
E. coli PHE10 88,260 mg/L
E. coli PHE12 92,210 mg/L
E. coli PHE11 94,720 mg/L
E. coli PHE13 101,580 mg/L
E. coli PHE17 103,150 mg/L
E. coli PHE14 105,360 mg/L

Biomass-specific titer in Flask for L-phenylalanine

Modifications
Strain Maximum production aroGEc aroFEc pheAEc tyrREc tyrAEc trpEEc csrAEc csrDEc csrBEc tktAEc zwfEc
E. coli NST37 -999,999,999
E. coli NSTCSRA -999,999,999
E. coli NSTCSRD -999,999,999
E. coli NST [pCsrB] -999,999,999
E. coli NSTCSRA [pCsrB] -999,999,999
E. coli NSTCSRACSRD -999,999,999
E. coli NST [pTktA] -999,999,999
E. coli NST [pZwf] -999,999,999
E. coli NSTCSRA [pTktA] -999,999,999
E. coli NSTCSRA [pZwf] -999,999,999
E. coli NSTCSRA [pTkaFpA] -999,999,999

Substrate yield in Flask for L-phenylalanine

Modifications
Strain Maximum production ptsHEc ptsIEc crrEc aroGEc tktAEc pheAEc aroFEc galPEc glkEc tyrREc ppsAEc pckEc talBEc aroDEc ydiBEc aroCEc
E. coli Xllp02 0.04 g/g of sustrate
E. coli Xllp15 0.04 g/g of sustrate
E. coli Xllp18 0.04 g/g of sustrate
E. coli JM101-fbr 0.05 g/g of sustrate
E. coli Xllp12 0.05 g/g of sustrate
E. coli Xllp14 0.05 g/g of sustrate
E. coli Xllp03 0.06 g/g of sustrate
E. coli Xllp09 0.06 g/g of sustrate
E. coli HD-1 0.07 g/g of sustrate
E. coli Xllp06 0.07 g/g of sustrate
E. coli Xllp10 0.07 g/g of sustrate
E. coli Xllp11 0.07 g/g of sustrate
E. coli Xllp19 0.07 g/g of sustrate
E. coli Xllp05 0.08 g/g of sustrate
E. coli Xllp13 0.08 g/g of sustrate
E. coli Xllp17 0.08 g/g of sustrate
E. coli Xllp04 0.09 g/g of sustrate
E. coli Xllp07 0.09 g/g of sustrate
E. coli Xllp20 0.11 g/g of sustrate
E. coli Xllp08 0.12 g/g of sustrate
E. coli Xllp23 0.13 g/g of sustrate
E. coli PB12-fbr 0.14 g/g of sustrate
E. coli Xllp22 0.14 g/g of sustrate
E. coli Xllp16 0.15 g/g of sustrate
E. coli Xllp21 0.16 g/g of sustrate
E. coli JM101-ev1 0.21 g/g of sustrate
E. coli JM101-ev2 0.22 g/g of sustrate
E. coli PB12-ev2 0.33 g/g of sustrate
E. coli PB12-ev1 0.33 g/g of sustrate

Substrate yield in Fed-Batch for L-phenylalanine

Modifications
Strain Maximum production tyrREc pheAEc aroFEc ptsHEc galPEc glkEc aroDEc dadXEc cvrAEc aroEEc xfpkBa ylbEEc pycCg pykFEc dhaLEc pckEc aspAEc mhpAEc adhEEc thrWEc ykfNEc
E. coli HD-1 0.186 g/g of sustrate
E. coli PHE09 0.206 g/g of sustrate
E. coli PHE07 0.207 g/g of sustrate
E. coli PHE10 0.207 g/g of sustrate
E. coli PHE08 0.208 g/g of sustrate
E. coli PHE11 0.222 g/g of sustrate
E. coli PHE12 0.227 g/g of sustrate
E. coli PHE17 0.229 g/g of sustrate
E. coli PHE13 0.229 g/g of sustrate
E. coli PHE14 0.229 g/g of sustrate
E. coli Xllp21 0.26 g/g of sustrate

Biomass-specific productivity in Flask for L-phenylalanine

Modifications
Strain Maximum production ptsHEc ptsIEc crrEc aroGEc tktAEc pheAEc
E. coli JM101-ev2 36 mg/L/g DCW
E. coli PB12-ev1 38 mg/L/g DCW
E. coli JM101-ev1 38 mg/L/g DCW
E. coli PB12-ev2 40.6 mg/L/g DCW