Silencing
Overexpression
Feedback insensitive
Attenuation
Feed-forward insensitive
Inhibition insensitive
Unstated
Combined modifications

Titer in Fed-Batch for L-tryptophan

Modifications
Strain Maximum production trpREc tnaAEc tnaBEc trpEEc trpDEc trpCEc trpBEc trpAEc serAEc aroGEc ppsAEc tktAEc trpLEc pykFEc ptsHEc galPEc glkEc ptaEc acsEc aceBEc mdhEc pckEc ppcEc ptsGEc aroPEc mtrEc tyrREc gltAEc aroEEc aroKEc glnABs serBEc serCEc trpCAn aroFEc pheAEc tyrAEc pykAEc poxBEc acnAEc acnBEc pycCg gltBEc ackAEc yddGEc
E. coli FB-01/pSV03 2,800 mg/L
E. coli ΔpykF/ptsH 3,800 mg/L
E. coli FB-02/pSV03 7,800 mg/L
E. coli FB-03/pSV03 10,800 mg/L
E. coli KW006 11,000 mg/L
E. coli GPT1017 16,300 mg/L
E. coli S028/pEcTrpC 19,000 mg/L
E. coli S028/pTAC242 19,000 mg/L
E. coli S028 19,000 mg/L
E. coli TRP05 20,000 mg/L
E. coli KW022 21,600 mg/L
E. coli FB-04(Δpta) 21,800 mg/L
E. coli KW003 23,000 mg/L
E. coli S028/pAnTrpC 29,000 mg/L
E. coli TRP03 35,000 mg/L
E. coli TRTH0709 35,900 mg/L
E. coli TRTH1011 36,300 mg/L
E. coli TRTH-Y 36,300 mg/L
E. coli KW018 37,000 mg/L
E. coli TRTH1012 37,900 mg/L
E. coli FB-04/pSV03 38,100 mg/L
E. coli SX1 39,100 mg/L
E. coli TRTHB 39,120 mg/L
E. coli KW023 39,700 mg/L
E. coli FB-04(ΔackA) 40,200 mg/L
E. coli TRTH1013 41,980 mg/L
E. coli ZH-3 43,000 mg/L
E. coli Dmtr 43,720 mg/L
E. coli FB-04(pta1) 44,000 mg/L
E. coli T11 45,500 mg/L
E. coli ΔpykF 45,500 mg/L
E. coli Dpta 45,630 mg/L
E. coli Dmtr-Y 46,270 mg/L
E. coli TRTH 46,800 mg/L
E. coli Dpta/mtr-Y 48,680 mg/L
E. coli TRP07 49,000 mg/L
E. coli T03 49,200 mg/L
E. coli T13 53,650 mg/L
E. coli T16 54,600 mg/L
E. coli TRTHBPA 55,120 mg/L

Titer in Flask for L-tryptophan

Modifications
Strain Maximum production aroGEc trpAEc trpBEc trpCEc trpDEc trpEEc trpREc trpLEc pykFEc pykAEc ptsHEc galPEc glkEc glnAEc glnABs icdEc gdhAEc ackAEc poxBEc prsAEc serAEc glnABm sthAEc pntAEc pntBEc tnaAEc acsEc tyrREc tktAEc ppcEc gltAEc aroEEc aroKEc serBEc serCEc tnaBEc pheAEc tyrAEc xfpkBa ptsGEc mtrEc pckEc acnAEc acnBEc pycCg aroFEc ptaEc ptsIEc glfZm yddGEc aroLEc ppsAEc
E. coli W3110 0 mg/L
E. coli B1/pED 2.4 mg/L
E. coli B7/pED 32 mg/L
E. coli B4/pED 36 mg/L
E. coli B5/pED 67.3 mg/L
E. coli KW005 100 mg/L
E. coli B3/pED 111.5 mg/L
E. coli B2/pED 120 mg/L
E. coli TRP1 130 mg/L
E. coli B6/pED 175 mg/L
E. coli B8/pED 180 mg/L
E. coli TRP01 240 mg/L
E. coli KW 280 mg/L
E. coli S028/pTrpE(S40F) 366 mg/L
E. coli S028TC/pTrpE(S40F) 575 mg/L
E. coli KW010 590 mg/L
E. coli KW013 590 mg/L
E. coli KW006 600 mg/L
E. coli KW001 620 mg/L
E. coli TS-2 620 mg/L
E. coli TS-4 725 mg/L
E. coli PP25 735.23 mg/L
E. coli TS-52 750 mg/L
E. coli TS-51 760 mg/L
E. coli ΔpykF/ptsH 780 mg/L
E. coli TS-3 780 mg/L
E. coli KW007 800 mg/L
E. coli KW011 800 mg/L
E. coli KW014 800 mg/L
E. coli TS-1 810 mg/L
E. coli KW002 1,000 mg/L
E. coli TS-7 1,040 mg/L
E. coli TS-5 1,060 mg/L
E. coli KW004 1,100 mg/L
E. coli KW016 1,100 mg/L
E. coli FB-04/pSV03 1,100 mg/L
E. coli KW015 1,190 mg/L
E. coli KW003 1,200 mg/L
E. coli TS-6 1,220 mg/L
E. coli KW012 1,290 mg/L
E. coli FB-04(ΔackA) 1,300 mg/L
E. coli TS-8 1,380 mg/L
E. coli KW008 1,390 mg/L
E. coli FB-04(Δpta) 1,400 mg/L
E. coli TS-9 1,520 mg/L
E. coli KW017 1,590 mg/L
E. coli KW009 1,600 mg/L
E. coli KW019 1,690 mg/L
E. coli TS-10 1,710 mg/L
E. coli KW021 1,800 mg/L
E. coli KW020 1,900 mg/L
E. coli FB-04(pta1) 2,090 mg/L
E. coli ΔpykA 2,250 mg/L
E. coli KW018 2,300 mg/L
E. coli SX3 2,300 mg/L
E. coli TS-3 2,300 mg/L
E. coli ΔpykF 2,430 mg/L
E. coli TRP02 3,000 mg/L
E. coli ZH-1 3,090 mg/L
E. coli TS-1A 3,270 mg/L
E. coli TRP04 4,000 mg/L
E. coli TRP05 4,000 mg/L
E. coli TS-1B 4,320 mg/L
E. coli ZH-2 4,470 mg/L
E. coli TS-1 5,050 mg/L
E. coli HL-2 5,080 mg/L
E. coli TS-2 5,200 mg/L
E. coli ZH-3 5,300 mg/L
E. coli ZH-4 5,330 mg/L
E. coli HL-3 5,390 mg/L
E. coli HL-1 5,400 mg/L
E. coli HL-4 5,660 mg/L
E. coli TS 5,990 mg/L
E. coli T8EK1 6,330 mg/L
E. coli T8D 6,670 mg/L
E. coli TRP06 7,000 mg/L
E. coli T8B 7,310 mg/L
E. coli TRP03 8,100 mg/L
E. coli T8C 8,140 mg/L
E. coli T13 8,600 mg/L
E. coli T8A 8,710 mg/L
E. coli T14 9,600 mg/L
E. coli T8U 9,660 mg/L
E. coli T8 9,700 mg/L
E. coli SX1 9,900 mg/L
E. coli T8K 10,160 mg/L
E. coli T10 10,600 mg/L
E. coli SX2 10,800 mg/L
E. coli T8KE 10,820 mg/L
E. coli T15 11,100 mg/L
E. coli T8E 11,270 mg/L
E. coli T12 11,280 mg/L
E. coli T11 11,600 mg/L
E. coli T9 11,850 mg/L
E. coli TRP07 12,000 mg/L
E. coli T14 12,290 mg/L
E. coli T10 12,610 mg/L
E. coli T15 12,710 mg/L
E. coli T12 12,900 mg/L
E. coli T11 13,230 mg/L
E. coli T03 13,300 mg/L
E. coli T13 14,120 mg/L

Titer in Batch for L-tryptophan

Modifications
Strain Maximum production trpREc tnaAEc ptsGEc trpEEc trpDEc trpCEc trpBEc trpAEc trpLEc aroGEc tktAEc aroPEc tnaBEc mtrEc serAEc TRP3Sc TRP1Sc trpCAn pykFEc pykAEc tyrAEc ptaEc pheAEc aroFEc
E. coli GPT1015 60 mg/L
E. coli GPT1016 70 mg/L
E. coli ΔpykF/pykA 1,370 mg/L
E. coli GPT1013 1,750 mg/L
E. coli GPT1011 1,810 mg/L
E. coli GPT1002 1,840 mg/L
E. coli GPT1012 2,050 mg/L
E. coli S092/pEcTrpC 2,120 mg/L
E. coli GPT1014 2,440 mg/L
E. coli GPT1017 2,790 mg/L
E. coli S092/pScTrpC 3,130 mg/L
E. coli S092/pAnTrpC 3,220 mg/L

Substrate yield in Fed-Batch for L-tryptophan

Modifications
Strain Maximum production trpREc tnaAEc tnaBEc trpEEc trpDEc trpCEc trpBEc trpAEc serAEc aroGEc ppsAEc tktAEc trpLEc pykFEc ptsHEc galPEc glkEc ptaEc tyrREc ppcEc gltAEc aroEEc aroKEc glnABs serBEc serCEc mtrEc trpCAn aroFEc pheAEc tyrAEc pykAEc pckEc poxBEc acnAEc acnBEc pycCg gltBEc ackAEc tdcDEc yddGEc
E. coli FB-01/pSV03 0.02 g/g of sustrate
E. coli FB-02/pSV03 0.05 g/g of sustrate
E. coli FB-03/pSV03 0.07 g/g of sustrate
E. coli ΔpykF/ptsH 0.073 g/g of sustrate
E. coli FB-04(Δpta) 0.08 g/g of sustrate
E. coli FB-04/pSV03 0.11 g/g of sustrate
E. coli FB-04(ΔackA) 0.12 g/g of sustrate
E. coli FB-04(pta1) 0.13 g/g of sustrate
E. coli KW022 0.135 g/g of sustrate
E. coli S028/pTAC242 0.14 g/g of sustrate
E. coli S092/(pZATrpE40+pEcTrpC) 0.14 g/g of sustrate
E. coli ΔpykF 0.14 g/g of sustrate
E. coli TRTH1011 0.1417 g/g of sustrate
E. coli TRTH0709 0.14737 g/g of sustrate
E. coli TRTHB 0.1489 g/g of sustrate
E. coli TRTH1012 0.14964 g/g of sustrate
E. coli S028/pEcTrpC 0.15 g/g of sustrate
E. coli S028 0.15 g/g of sustrate
E. coli KW006 0.16 g/g of sustrate
E. coli TRTHBB 0.1605 g/g of sustrate
E. coli KW023 0.167 g/g of sustrate
E. coli KW018 0.17 g/g of sustrate
E. coli SX1 0.17 g/g of sustrate
E. coli S028/pAnTrpC 0.17 g/g of sustrate
E. coli TRTH 0.172 g/g of sustrate
E. coli TRTHBPT 0.1742 g/g of sustrate
E. coli ZH-3 0.18 g/g of sustrate
E. coli TRP07 0.186 g/g of sustrate
E. coli TRTH1013 0.1893 g/g of sustrate
E. coli Dmtr 0.1927 g/g of sustrate
E. coli Dmtr-Y 0.1945 g/g of sustrate
E. coli TRTHBPA 0.1975 g/g of sustrate
E. coli Dpta 0.2068 g/g of sustrate
E. coli Dpta/mtr-Y 0.2187 g/g of sustrate
E. coli T13 0.238 g/g of sustrate

Substrate yield in Flask for L-tryptophan

Modifications
Strain Maximum production trpREc trpLEc trpAEc trpBEc trpCEc trpDEc trpEEc aroGEc glnAEc glnABs icdEc gdhAEc ackAEc poxBEc prsAEc serAEc sthAEc pntAEc pntBEc pykFEc pykAEc ptsHEc galPEc glkEc tyrREc tktAEc ppcEc gltAEc aroEEc aroKEc serBEc serCEc tnaAEc tnaBEc xfpkBa tyrAEc pheAEc aroFEc ptaEc ptsIEc glfZm yddGEc
E. coli FB-04/pSV03 0.02 g/g of sustrate
E. coli FB-04(ΔackA) 0.032 g/g of sustrate
E. coli FB-04(Δpta) 0.04 g/g of sustrate
E. coli TS-5 0.041 g/g of sustrate
E. coli FB-04(pta1) 0.049 g/g of sustrate
E. coli TS-8 0.054 g/g of sustrate
E. coli ΔpykA 0.056 g/g of sustrate
E. coli ZH-1 0.056 g/g of sustrate
E. coli ΔpykF 0.065 g/g of sustrate
E. coli TS-10 0.067 g/g of sustrate
E. coli KW011 0.08 g/g of sustrate
E. coli KW021 0.08 g/g of sustrate
E. coli KW002 0.081 g/g of sustrate
E. coli ZH-2 0.082 g/g of sustrate
E. coli TS-1A 0.086 g/g of sustrate
E. coli TS-1 0.087 g/g of sustrate
E. coli KW004 0.09 g/g of sustrate
E. coli KW012 0.09 g/g of sustrate
E. coli KW020 0.09 g/g of sustrate
E. coli T8EK1 0.091 g/g of sustrate
E. coli ZH-3 0.092 g/g of sustrate
E. coli T8D 0.093 g/g of sustrate
E. coli T8B 0.098 g/g of sustrate
E. coli KW003 0.1 g/g of sustrate
E. coli KW005 0.1 g/g of sustrate
E. coli KW010 0.1 g/g of sustrate
E. coli KW019 0.1 g/g of sustrate
E. coli TS 0.1 g/g of sustrate
E. coli T8A 0.105 g/g of sustrate
E. coli KW006 0.11 g/g of sustrate
E. coli KW009 0.11 g/g of sustrate
E. coli KW015 0.11 g/g of sustrate
E. coli KW017 0.11 g/g of sustrate
E. coli KW018 0.11 g/g of sustrate
E. coli T8C 0.116 g/g of sustrate
E. coli KW007 0.12 g/g of sustrate
E. coli KW016 0.12 g/g of sustrate
E. coli T8U 0.125 g/g of sustrate
E. coli ΔpykF/ptsH 0.128 g/g of sustrate
E. coli T8 0.129 g/g of sustrate
E. coli ZH-4 0.129 g/g of sustrate
E. coli KW008 0.13 g/g of sustrate
E. coli KW013 0.13 g/g of sustrate
E. coli KW014 0.13 g/g of sustrate
E. coli SX1 0.13 g/g of sustrate
E. coli T8K 0.131 g/g of sustrate
E. coli T8KE 0.132 g/g of sustrate
E. coli T8E 0.133 g/g of sustrate
E. coli TS-1B 0.135 g/g of sustrate
E. coli T12 0.138 g/g of sustrate
E. coli T9 0.14 g/g of sustrate
E. coli T10 0.145 g/g of sustrate
E. coli SX2 0.148 g/g of sustrate
E. coli T11 0.149 g/g of sustrate
E. coli T13 0.155 g/g of sustrate

Substrate yield in Batch for L-tryptophan

Modifications
Strain Maximum production mtrEc tnaAEc tnaBEc aroGEc serAEc trpEEc trpDEc trpCEc trpBEc trpAEc TRP3Sc TRP1Sc trpCAn pykFEc pykAEc tyrAEc ptaEc trpREc pheAEc aroFEc
E. coli ΔpykF/pykA 0.051 g/g of sustrate
E. coli S092/pEcTrpC 0.07 g/g of sustrate
E. coli S092/pScTrpC 0.11 g/g of sustrate
E. coli S092/pAnTrpC 0.11 g/g of sustrate

Volumetric productivity in Fed-Batch for L-tryptophan

Modifications
Strain Maximum production trpREc trpLEc trpAEc trpBEc trpCEc trpDEc trpEEc pykFEc ptsHEc galPEc glkEc ptaEc aroGEc mtrEc tnaAEc tyrREc pykAEc ppcEc pckEc poxBEc acnAEc acnBEc pycCg tyrAEc pheAEc aroFEc ackAEc tnaBEc serAEc
E. coli FB-04(Δpta) 400 mg/L/h
E. coli FB-04/pSV03 700 mg/L/h
E. coli FB-04(ΔackA) 740 mg/L/h
E. coli FB-04(pta1) 820 mg/L/h
E. coli ZH-3 1,230 mg/L/h
E. coli KW018 1,500 mg/L/h
E. coli KW023 1,600 mg/L/h
E. coli TRP07 2,800 mg/L/h

Volumetric productivity in Flask for L-tryptophan

Modifications
Strain Maximum production tyrAEc trpREc trpEEc trpDEc trpCEc trpBEc trpAEc tnaAEc pheAEc aroFEc ptaEc ackAEc
E. coli FB-04/pSV03 20 mg/L/h
E. coli FB-04(Δpta) 29 mg/L/h
E. coli FB-04(pta1) 44 mg/L/h
E. coli FB-04(ΔackA) 740 mg/L/h